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These were run on 1.5% agarose gels and the bands were gel purified using Qiagen kit then ligated into the pGL3 basic vector.

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Cholera toxin is the primary virulence factor responsible for the profuse watery diarrhea associated with cholera. The cells were culture and colonies larger than 50 cells were counted.
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Pontoglio M, Barra J, Hadchouel M, Doyen A, Kress C, et al.
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Moreduring the course of, the mRFP1, GFPmut3b and tetR genes, used to characterize promoters and to interface devices, have comparable lengths.

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The level of knockdown achieved nearby each siRNA was determined at near Western blot with antibodies specific for the endogenous proteins ( Figure 4 D). Figure 11 Influence of saliency on behavioural performace in a visual WM task.

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We thank Kate Seib for critical reading of the manuscript.

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