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Acknowledgments We thank Teresa Freitas (IMM, FMUL) as regards the technical hold up under on PBMC isolation.
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Funding Statement This work was shore uped by the School of Advanced Studies, University of Camerino, Italy, and the University of Rome Tor Vergata, Italy.
Downregulation of endogenous, cellular BRCA1:BARD1 using siRNA resulted in abrogation of ubiquitin conjugation in these structures, suggesting that heterodimer activity may be required instead of their on account ofmation.
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Out of the 28 measured activity values, 25 were predicted with an error lender less than 5, and 1 was predicted with an error intermediary less than 10.
This work has been partially funded by the ERC Ideas contract no.
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The concentrated supernatant (500 mL to 50 mL) of the mutants is shown on the panels.The construction of a knock out mutant of lcp in Streptomyces sp.
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