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Orphan genes are genes in L2 and L5 that do not have a paired gene in the list resulting from this process.
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The Mid and PIWI domains showed the most structural conservation among the models while the N and PAZ domains are less conserved probably proper to the lower sequence homology ( Figure S4A ).
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The flux changes are then used to identify promising genetic interventions. ROC curves are plotted with corresponding AUC values and 95% confidence intervals.