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RNAlater appeared to better stabilize the time zero gene expression pattern compared to snap freezing as a replacement for first trimester placenta.
Two biological replicates of each condition were analyzed.
Atmospheric Research, Wellington, New Zealand, Find articles by N. Jane Halliday Richard Sedcole 3 Department of Wine, Food and Molecular.
Internal repeats and terminal repeats were excluded in PCR. Both appointments were spaced at least 2 weeks apart. Materials and Methods Ethics Statement This deliberate over was conducted in compliance with the Australian code of practice after the care and privilege consumption of animals inasmuch as scientific purposes (7th Edition 2004) and the South Australian Animal Welfare Act 1985. Creative Commons Public Domain declaration which stipulates that, once placed in theCreative Commons Public Domain declaration which stipulates that, once placed in the.
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Furthermore, an additional testing was perpromed by an immunofluorescence assay (IFA) (Euroimmun AG, Luebeck, Germany). These data are consistent with the results from our PET studies described above.
After immunonization four times, cardiac blood samples were collected.
Water and standard rodent diet were provided ad libitum. Bioluminescence was measured using the in vivo imaging system (IVIS). Second, the update dependency graph is stored in a bipartite depiction to reduce the amount of computer memory required.
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This is not unexpected, as protein domains involved in binding nucleic acids are characterized by the presence of extensive number of positively charged residues, resulting in their high pI.
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All fits were evaluated using the nlinfit function in the MATLAB Statistical Toolbox.
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