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Total RNA isolation and cDNA synthesis Each sample was homogenized into powder in presence of liquid nitrogen, followed by total RNA extraction with Trizol (Invitrogen Corporation, Carlsbad, CA, USA), and the quality of the total RNA was checked with the Agilent 2100 bioanalyser (Agilent Technologies, Santa Clara, CA, USA). Pregnancy is known to be a state of transient hypercortisolemia (135).
All plots were based on the relaxed clock assumption.
Wang C, Vlahov D, Galai N, Cole SR, Bareta J, et al. (2005) The effect of HIV infection on overdose mortality. AIDS.
Siddiqi SH, Libonati JP, Middlebrook G. Evaluation of rapid radiometric method for drug susceptibility testing of Mycobacterium tuberculosis.
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