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Primary antibodies were detected using secondary antibodies conjugated to Alexa fluorophores (Invitrogen).
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Figure 8 Sensitivity of the modules with respect to the size of the dataset.
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To investigate general patterns in IDs, we determined the percentage of IDs per predefined ID category (see Table 1 ).
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Injection of experimental MO resulted in strong inhibition of substrate cleavage ( Figure 8B ) and the majority of MO injected embryos in a matched hatching assay did not hatch ( Figure 8B, legend).
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